Standardization of Methyl Green for Specific Staining of Egg-shell Material in a Trematode By
نویسنده
چکیده
I . The specificity of a number of samples of methyl green for egg-shell material in Fasciola hepatica was investigated. 2. A method for distinguishing between specific and non-specific stains by paper chromatography is described. 3. Specific stains gave almost identical chromatograms with an Rf value of 083 for both green and violet constituents. Non-specific samples gave chromatograms with an Rf of 065 for the green constituent and an Rf of 0-84 for the violet constituent. The liquid phase used was a mixture of organic solvents in the following proportions: ethanol, 100; water, 80; tt-butanol, 100; cellosolve, 100. 4. The application of this technique has led to the production of a methyl green, named Methyl Green M.2, specific for shell material in Fasciola.
منابع مشابه
A clean and efficient synthesis of spiro[4H-pyran-oxindole] derivatives catalyzed by egg shell
Egg shell has been utilized as a natural, green, reusable and eco-friendly reagent for the synthesis of spiro[4H-pyran-oxindole] derivatives by one-pot multicomponent reaction of isatins, 1,3-diketones, and malononitrile/ ethyl cyanoacetate. Good functional group tolerance and broad scope of usable substrates are other prominent features of the present methodology.
متن کاملHazelnut shell as a valuable bio-waste support for green synthesis of Ag NPs using Origanum vulgare leaf extract: Catalytic activity for reduction of methyl orange and Congo red
In this work the Origanum vulgare leaf extract was used to green synthesis of Ag nanoparticles (NPs) supported on Hazelnut shell as an environmentally benign support. The Ag NPs/Hazelnut shell as an effective catalyst was prepared through reduction of Ag+ ions using Origanum vulgare leaf extract as the reducing and capping agent and Ag NPs immobilization on Hazelnut shell surface in ...
متن کاملDetection of Apoptosis in Leukoplakia and Oral Squamous Cell Carcinoma using Methyl Green Pyronin and Hematoxylin and Eosin
Background & Objective: Cell population and turnover are controlled by a balance between cell proliferation and apoptosis. Detection of apoptosis in oral cancer contributes to its better prognosis and improved management. This study aimed to quantify apoptotic cells in leukoplakia and oral squamous cell carcinoma (OSCC) using methyl green-pyronin (MGP) and hematoxylin and eosin...
متن کاملHazelnut shell as a valuable bio-waste support for green synthesis of Ag NPs using Origanum vulgare leaf extract: Catalytic activity for reduction of methyl orange and Congo red
In this work the Origanum vulgare leaf extract was used to green synthesis of Ag nanoparticles (NPs) supported on Hazelnut shell as an environmentally benign support. The Ag NPs/Hazelnut shell as an effective catalyst was prepared through reduction of Ag+ ions using Origanum vulgare leaf extract as the reducing and capping agent and Ag NPs immobilization on Hazelnut shell surface in ...
متن کاملComparative Assessment of Nuclear and Nucleolar Cytochemical Parameters of Oral Epithelial Cells in Smokers and Non-Smokers by Methyl Green-Pyronin Staining
Introduction: A strong relationship exists between cigarette smoking and the development of oral squamous cell carcinoma. Smoking can significantly increase cellular proliferation. Nevertheless, there is little reference in literature to the cytological assessment of oral mucosa in this respect. Methods: Changes in nuclear and neucleolar cytomorphometric parameters such as diameter, surface, nu...
متن کامل